Systematic analysis of overall survival and interactions between tumor mutations and drug treatment
© Gatto and Nielsen. 2016
Received: 28 January 2016
Accepted: 24 February 2016
Published: 2 March 2016
Few exceptional responses in cancer treatment were attributed to a genetic predisposition of the tumor.
We analyzed a cohort of 3105 patients from 12 different cancer types and systematically sought the existence of a correlation between overall survival and the interaction of 21 antineoplastic treatments with 6 tumor mutations.
We identified a single significant correlation resulting in increased overall survival from temozolomide in lower-grade glioma with IDH1 R132H mutations. The trend could not be attributed to either the treatment or the mutation alone. Univariate and multivariate Cox regression demonstrated that this interaction stood as an independent prognostic predictor of survival.
Our results suggest infrequent instances of exceptional responses ascribable to tumor genomics yet corroborate the existence of an interaction of temozolomide with IDH1 mutations in lower-grade glioma.
The cancer genome can elicit sensitivity to certain drugs not specifically designed to target the underlying genetic aberrations. To this end, genomic markers of drug sensitivity have been systematically assessed in cancer cell lines [1, 2]. Ideally, these markers can identify patients who may better benefit from a certain antineoplastic drug [3, 4]. In contrast to the increasing availability of data about genomics of drug sensitivity in vitro , the association with improved patient survival is so far limited to few clinical cases, e.g., exceptional responses to everolimus in bladder cancers with TSC1 mutations .
Here, we sought to systematically assess if the chances of overall survival in patients with a certain cancer type and treated with a given antineoplastic drug correlate with the presence of a certain genetic mutation in the tumor. The examined cohort comprised 3105 patients, spanning 12 cancer types (with 81–731 samples for each cancer type). Collectively, 21 antineoplastic drugs were administered each in at least 20 patients (median 82; IQR 29–150). Six cancer-associated mutations were detected in at least 20 patients in this cohort: V600E in BRAF (n = 29), R132H in IDH1 (n = 108), G12V in KRAS (n = 49), H1047R in PIK3CA (n = 89), R175H in TP53 (n = 45), and V777 deletion in ZFHX3 (n = 22). After binning samples by cancer type, out of 1512 potential associations, 9 associations between overall survival, drug treatment, and tumor mutation had sufficient sample size for each covariate and were hereby tested. The hazard ratio (HR) for each interaction between drug treatment and tumor mutation in a cancer type was estimated in a multivariate analysis using a nested Cox proportional hazard regression model. We adopted a likelihood ratio test to test whether there is a significant effect of the interaction on overall survival on top of the tumor mutation and administered drug alone (Additional file 1: Table S1).
Hazard ratio (HR) for clinical factors in the overall survival of lower-grade glioma
N [n death]
95 % CI
95 % CI
R132H in IDH1
Supratentorial, frontal lobe
Supratentorial, occipital lobe
Supratentorial, parietal lobe
Supratentorial, temporal lobe
Symptoms at diagnosis
Mental status changes
In conclusion, we identified one genomic marker of drug sensitivity that was associated with better survival in patients, in contrast to patients treated with the same drug but with no detected mutation or vice versa. Indeed, mutations in IDH1 were previously implicated with good prognosis in brain tumors treated with TMZ [8, 9]. Our results independently validate these findings and further extend the reach of this correlation beyond some previous limitations . First and foremost, the cohort size allowed discerning that an increase in patient survival was exquisitely associated with the interaction between IDH1 mutations and TMZ, suggestive of a synergy between treatment and tumor genomics. Second, it specifically correlated with R132H mutations. Finally, we recovered a negative time-dependent effect of the interaction, which is reminiscent of emergence of drug resistance and in line with the genetic evolution of lower-grade glioma attributed to TMZ treatment .
The authors acknowledge Knut and Alice Wallenberg Foundation for financing this work.
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