Fig. 1

M2 macrophages are increased in BM of MM-inoculated mice. A Schematic representation of xenograft systemic model of CXCR4-driven MM with BM involvement. NSG mice were i.v. inoculated with RPMI8226-CXCR4-GFP cells. Non-injected NSG mice were used as controls. B MM tumor load was measured by enumeration of GFP+ human MM cells in the BM and by evaluation of human IgG levels in the blood of non-injected (n = 5) or inoculated animals (n = 5). Data are presented as mean ± SE, **p < 0.01. C Presence of M2 murine MΦ in the BM of non-injected controls (n = 5) or MM-inoculated animals (n = 5), evaluated by flow cytometry. Representative plots for gating strategy evaluating the expression of murine CD11b, F4/80, CD206, and MERTK markers. Bars showing mean of triplicates ± STDEV, **p < 0.01