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Fig. 3 | Journal of Hematology & Oncology

Fig. 3

From: KAI1(CD82) is a key molecule to control angiogenesis and switch angiogenic milieu to quiescent state

Fig. 3

Anti-angiogenic LIF is the downstream effect molecule induced by KAI1. a GSEA of the “negative angiogenic regulators” (GO: 0016525) and “blood vessel remodelling” (GO: 0001974), based on the RNA sequencing of WT and Kai1−/− PC. b Venn diagram showing the leading edge subset (core enrichment genes) of GSEA data for “negative angiogenic regulators” (GO: 0016525) and “blood vessel remodelling” (GO: 0001974), based on the RNA sequencing and semi-quantitative RT-PCR of WT and Kai1−/− PC. c qRT-PCR analysis for Kai1 mRNA level in Kai1-O/E ECs (MS1) and PCs (10T1/2). The genes were normalized by Gapdh (**p < 0.05, Means ± SEM, 3 technical replicates). d Lif expression in the Kai1-O/E MS1 and 10T1/2 cells (Means ± SEM, 3 technical replicates). e Lif level determination in the conditioned media of Kai1-O/E MS1 and 10T1/2 cells (Means ± SEM, 5 technical replicates). f Top, Tube formation assay using MS1 cells cultured in the conditioned media obtained from the mock and Kai1-O/E 10T1/2. These media were treated with control IgG or Lif antibody. Scale bar, 50 μm. Bottom, Quantification of the obtained results (*p < 0.01, **p < 0.05, ***p < 0.001, Means ± SEM, 3 technical replicates). g Changes in the levels of angiogenic molecules (Ang2, Esm1, Dll4, Vegfr2, Sox7, Sox17, and Sox18) by using semi-quantitative RT-PCR in MS1 cells following the treatment with mouse Lif (10 and 100 ng/mL). ns; not significant, **p < 0.05, ***p < 0.001. h Changes in Vegfa, Fgf2, Pdgfbb, Vegfr2, Dll4 and Ang2 levels in 10T1/2 cells in response to the Lif treatment (10 and 100 ng/mL). ns; not significant, *p < 0.01, **p < 0.05, ***p < 0.001. i After palmitoylation inhibition with 2-bp in 10T1/2 cells, Lif expression was detected by qRT-PCR. ***p < 0.001

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