Fig. 6

FUBP1 knockdown inhibits proliferation, migration, and the cell cycle of lung cancer cells. A qRT-PCR analysis of FUBP1 expression in lung cancer cells transfected with FUBP1 siRNA (siRNA1 or siRNA2) or NC siRNA. B Western blot analysis of FUBP1 expression in lung cancer cells transfected with FUBP1 siRNA or NC siRNA. C CCK-8 assays for A549 and Calu1 cells transfected with FUBP1 siRNA or NC siRNA. D Colony formation assays for A549 and Calu1 cells transfected with FUBP1 siRNA or NC siRNA. E, F Transwell migration assays for FUBP1-silenced and control lung cancer cells. Representative images (E) and statistical analysis of three independent assays (F) are shown. G Western blot analysis of cyclin A2 and cyclin D1 in A549 and Calu1 cells transfected with FUBP1 siRNA or negative control siRNA. H, I Cell cycle analysis by flow cytometry was performed to determine the percentage of cells in different cell cycle phases (H). Bar chart showing the percentage of cells in G1–G0, S, or G2–M phase (I)