Fig. 6

Tagln2^−/− DCs do not fully support T cell adhesion in vitro and in vivo. a SEM image of a representative DC-T cell interaction. WT or Tagln2^−/− DCs were pulsed with pOVA (323–339) and co-incubated with OTII CD4⁺ T cells for 1 h. Scale bar, 5 μm. b Reduced conjugate formation in Tagln2^−/− DCs. Stable conjugates were identified as double-positive events by flow cytometry. c Schematic diagram of the experimental setup for c and d. Representative cryosection images showing the overall distribution of WT or Tagln2^−/− DCs (green) and OTII CD4⁺ T cells (red) in draining lymph nodes. The statistical analysis of the number of migrated CD4⁺ T cells or DCs was performed. Scale bar, 100 μm. d Representative snapshot of live images of DC–T cell interactions in vivo. Draining popliteal LNs were visualized by two-photon microscopy. Scale bar, 10 μm. White arrowheads indicate the contact cells. Statistical analysis of the number of T cells in contact with one DC, the contact duration, and the T cell speed are presented. All data from b–d represent the mean of three experiments ± SEM. *P < 0.05