Fig. 6
From: Discovery of a dual inhibitor of NQO1 and GSTP1 for treating glioblastoma
MNPC decreases cell proliferation by overexpressing NQO1 and GSTP1. a MTT assay of U87MG/EGFRvIII cells. U87MG/EGFRvIII cells were overexpressed either NQO1 or GSTP1 or both, followed by treatment with MNPC. Cell treated with MNPC (1 μM) or Myc-DDK-GSTP1 or HA-NQO1 or combination for 5 days. b The ROS levels, c LDH levels, d carbonyl expression levels and e GSH/GSSG ratios of U87MG/EGFRvIII cells overexpressing NQO1 and GSTP1, followed by MNPC treatment. f Western blot analysis of U87MG/EGFRvIII cells treated with MNPC (1 μM) or Myc-DDK-GSTP1 or HA-NQO1 or combination for 48 h. g Effect of overexpression on apoptosis confirmed by TUNEL assay. The images of TUNEL-positive cells were captured by a confocal microscope. Scale bar: 20 μm. Data are means ± SEM (*p < 0.05; **p < 0.01; ***p < 0.001, n = 3)