Fig. 6

LINC01234 represses BTG2 expression by binding to EZH2 and LSD1 in NSCLC cells. a qRT-PCR analysis of mRNA levels of selected genes in LINC01234-depleted A549 and SPC-A1 cells compared with control cells. b Western blot analysis of BTG2 protein levels in A549 and SPC-A1 cells depleted of LINC01234. c qRT-PCR and Western blot analysis of BTG2 and EZH2 expression in A549 and SPC-A1 cells transfected with control (NC) siRNA and either EZH2 siRNA. d qRT-PCR and western blot analysis of BTG2 and LSD1 expression in A549 and SPC-A1 cells transfected with si-NC and either LSD1 siRNA. e ChIP-qPCR assay showing EZH2 and LSD1 occupancy on the BTG2 promoters and reduction by LINC01234 knockdown. f Immunostaining of BTG2 in NSCLC and normal tissues. g Kaplan–Meier survival plots of OS based on BTG2 expression in lung cancer patients (n = 1928). h Correlation analysis of LINC01234 expression and BTG2 expression. *P < 0.05, **P < 0.01