Fig. 4

VAV3 is a key target of miR-340-5p and miR-27b-3p in NSCLC cells. a Hierarchically clustered heatmap of mRNAs upregulated and downregulated in A549 cells after transfection of LINC01234 or control (NC) siRNAs. b Genes regulated by miR-27b-3p, miR-340-5p, and LINC01234 based on miRDB database and RNA-seq data analysis. c Predicted binding sites for miR-340-5p and miR-27b-3p in VAV3 mRNA. Luciferase activities were measured in NSCLC cells co-transfected with luciferase reporter containing VAV3 wild-type or mutant and the mimics of miR-340-5p and miR-27b-3p. d VAV3 mRNA levels in NSCLC cells depleted of miR-340-5p or miR-27b-3p. e Western blot analysis of E-cadherin, Vimentin and VAV3 expression in NSCLC cells transfected with VAV3 siRNAs. f Western blot and qRT-PCR analysis of VAV3 expression in LINC01234-depleted NSCLC cells. g Luciferase activities were measured in NSCLC cells co-transfected with luciferase reporter containing LINC01234 wild-type or mutant type, VAV3 wild-type, mimics of miR-340-5p and miR-27b-3p. h VAV3 protein levels in NSCLC cells co-transfected with LINC01234 siRNA and miR-340-5p or miR-27b-3p inhibitors. (I) Correlations between VAV3, miR-340-5p and miR-27b-3p expression. Correlation analysis of LINC01234 expression and VAV3 expression. *P < 0.05, **P < 0.01