Fig. 3

Clinical activity and expansion of haplo-TanCAR-T 19/22 cells. a There were 0.73% residual marrow blasts with expression of CD22 but loss of CD19 expression at day 14 after infusion, which were undetected by day 28. The cells in the D gate represent the blast population count of the total nucleated cells in BM aspirates. Day 0 is the day of haplo-TanCAR-T 19/22 cell infusion. b BM aspirates before and after haplo-TanCAR-T 19/22 cell infusion. Within the first 28 days after infusion, and at serial time points thereafter for 14 months, there was no evidence of blasts in BM. c, d Haplo-TanCAR-T 19/22 cells expanded within the first 12 days and continue to be detectable by flow cytometry with low levels in PB and BM through more than 14 months. B cells had not recovered as the most recent follow-up. e The presence of haplo-TanCAR-T 19/22 cells in PB and BM as assessed by qPCR