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Fig. 3 | Journal of Hematology & Oncology

Fig. 3

From: Downregulation of RNF128 activates Wnt/β-catenin signaling to induce cellular EMT and stemness via CD44 and CTTN ubiquitination in melanoma

Fig. 3

Downregulation of RNF128 induces EMT and stemness in melanoma cells. a The cellular morphologies in M14-shNC/M14-shRNF128 and A2058-Vector/A2058-RNF128 cells. b Western blot (left) and qRT-PCR (right) analyses were used to detect the expression of EMT markers (E-cadherin, vimentin, and Snail) and stemness markers (CD133) in M14-shNC/M14-shRNF128 and A2058-Vector/A2058-RNF128 cells. c The expression and location of RNF128, E-cadherin, vimentin, Snail, and CD133 markers in M14-shNC/M14-shRNF128 and A2058-Vector/A2058-RNF128 cells were analyzed by immunofluorescence staining. d Flow cytometric analyses were used to detect the expression of CD133 in the indicated cells. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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