Fig. 2

RNF128 downregulation promotes melanoma progression both in vitro and in vivo. a The efficiencies of RNF128 inhibition and overexpression were examined by western blot and qRT-PCR. b The effects of RNF128 inhibition and overexpression on cellular apoptosis were detected by flow cytometric analyses. c, d The effects of RNF128 knockdown or overexpression on cellular invasion and migration were measured by wound-healing migration assays (c) and Matrigel invasion assays (d). e The effects of RNF128 inhibition and overexpression on colony-forming ability were detected by colony formation assays. f The effects of RNF128 knockdown or overexpression on sphere-forming ability were detected by sphere-forming assays. g The effects of RNF128 inhibition and overexpression on lung metastasis were investigated in vivo assays, and the number of metastases was examined by H&E staining. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001