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Fig. 5 | Journal of Hematology & Oncology

Fig. 5

From: A potent immunomodulatory role of exosomes derived from mesenchymal stromal cells in preventing cGVHD

Fig. 5

MSCs-exo regulated the expression of transcription factors and inhibited the production of pro-inflammatory cytokines in vivo. ad Real-time PCR was utilized to analyze the mRNA expression of transcription factors in PBMC samples from cGVHD mice treated with PBS, Fib-exo, or MSCs-exo. There was no significant difference in gene expression between the PBS and Fib-exo groups, while the MSCs-exo group presented a remarkably reduced expression of RORrt (a) and STAT3 (b), both of which are involved in the development of Th17 cells, whereas the regulatory gene of Foxp3 (c) was increased. No significant difference in T-bet (d) gene expression was observed. ej A luminex assay was performed to detect the expression level of relevant cytokines. There were significant reductions of IL-17 (a), IL-21 (b), and IL-2 (i) in MSCs-exo-treated mice compared with the controls, while a slight decrease in IL-22 was observed (g). By contrast, IL-10 (h) expression was increased in the MSCs-exo group. Data are expressed as the mean ± SEM. *P < .05, **P < .01, and ***P < .001; ns not significant

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