Skip to main content
Fig. 4 | Journal of Hematology & Oncology

Fig. 4

From: A potent immunomodulatory role of exosomes derived from mesenchymal stromal cells in preventing cGVHD

Fig. 4

MSCs-exo inhibited IL-17-expressing pathogenic T cells and induced IL-10-expressing regulatory T cells in cGVHD. a Splenocytes from individual group mice were isolated and analyzed via an intracellular cytokine staining assay. The numbers in quadrants indicated the percent of CD4+ T cells expressing IL-17. There was a significant reduction of CD4 + IL-17A+ cells in MSCs-exo mice compared with the controls. b The lymphocytes from LNs were also analyzed, and the results indicated that MSCs-exo significantly suppressed the development of CD4 + IL-17A+ cells. c Treg cells in draining LNs were also analyzed by flow cytometry. As expected, the percentage of IL-10-expressing Treg cells was significantly increased in MSCs-exo-treated mice. d The expression of Foxp3, the critical transcription factor of Treg, was also analyzed. There was an obvious induction of Foxp3 expression in the lymphocytes from MSCs-exo-treated mice. Data are expressed as the mean ± SEM. *P < .05, **P < .01, and ***P < .001; ns not significant

Back to article page