Table 2 Culture systems of multiple tumoroids
From: Organoid technology and applications in cancer research
Tumoroid type | Culture components | Ref | ||
|---|---|---|---|---|
Extracellular matrix | Growth factors | Molecule inhibitors | ||
Stomach cancer | Matrigel (growth factor reduced) | ADMEM/F12, penicillin/streptomycin, L-glutamine, B27, N2, bovine serum albumin, EGF, Noggin, R-spondin-1, gastrin, FGF10, FGF-basic, Wnt3A, prostaglandin E2, and nicotinamide | A-83-01 Y27632 SB202190 | [26] |
Intestinal cancer | ||||
Liver cancer | Basement membrane extract | Classical human liver organoid isolation medium: ADMEM/F12, penicillin/streptomycin, GlutaMAX, HEPES, B27 (without vitamin A), N2, N-acetylcysteine, nicotinamide, gastrin 1, EGF, FGF10, HGF, forskolin, R-spondin-1, Wnt3A, and Noggin Tumoroid-specific isolation medium: Classical human liver organoid isolation medium with the elimination of R-spondin-1, Wnt3A, and Noggin as well as addition of dexamethasone Human healthy liver-derived organoids expansion medium Classical human liver organoid isolation medium with the elimination of Y27632, Wnt3A, and Noggin | A-83-01 Y27632 | [34] |
Pancreatic cancer | Matrigel | ADMEM/F12, penicillin/streptomycin, GlutaMAX, HEPES, B27, N-acetylcysteine, EGF, R-spondin-1, gastrin 1, Wnt3A, Noggin, and FGF | A-83-01 | [66] |
Breast cancer | Basement membrane extract (reduced growth factor) | ADMEM/F12, penicillin/streptomycin, GlutaMAX, HEPES, B27, N-acetylcysteine, R-spondin-1, FGF7, FGF10, nicotinamide, Noggin, primocin, and neuregulin 1 | A-83-01 Y27632 | [38] |
Bladder cancer | Matrigel | Hepatocyte media with EGF, FBS, GlutaMAX, and primocin | Y27632 | [67] |
Prostate cancer | Matrigel (growth factor reduced) | ADMEM, penicillin/streptomycin, primocin, GlutaMAX, B27, EGF, N-acetylcysteine, FGF10, FGF-basic, nicotinamide, testosterone, prostaglandin E2, Noggin, and R-spondin | A-83-01 SB202190 | [68] |