Fig. 1
From: Simple deep sequencing-based post-remission MRD surveillance predicts clinical relapse in B-ALL
Determination of optimal DNA input and analytical sensitivity. a. Effect of DNA input amount on assay sensitivity. b Linearity and sensitivity of the assay. Tenfold serial dilution of B-ALL diagnostic DNA samples (a 3623; b 3501 and 3064) with peripheral blood DNA from healthy individuals was performed, followed by deep VDJ sequencing procedure using a total DNA input of indicated amounts (a) or 5 μg (b) per sample. With 5 μg DNA input, the sensitivity of the assay can reach 10− 6