Skip to main content
Fig. 4 | Journal of Hematology & Oncology

Fig. 4

From: The CCR4-NOT complex is a tumor suppressor in Drosophila melanogaster eye cancer models

Fig. 4

The Not3 Not-box domain is important for normal protein functionality. a Qualitative and quantitative representation of the tumor burden upon downregulation of different CCR4-NOT complex subunits on the sensitized background. Bars show the percentage of eyes screened: blue, normal eyes; orange, hyperplastic eyes; ** p < 0.01; *** p < 0.001. The mean number of eyes screened is shown on the graph (Y-axis) and three independent crosses were established. Microcopy images show the eyes of adult flies from representative genotypes. Scale bars are 200 μM. Black arrow indicates the presence of metastasis. b Quantitative representation of the whole eye area (region with presence of differentiated photoreceptors) on representative adult eyes (n = 8) with downregulation of Not3 or twin on the sensitized genetic background. Control eyes measured values were considered as 100%; ns, not significant; ** p < 0.01; *** p < 0.001. c Schematic representation of the human CNOT3 and the fly Not3 proteins. ClustalW2 alignment analyses show that the coiled-coil (green) and Not-box (orange) regions are present in both species. Designed UAS constructs are also shown: UAS-Not3 (wild-type Not3), UAS-Not3 ΔCC (deletion of the coiled-coil domain region), UAS-Not3 ΔNB (deletion of the Not-box domain). d Qualitative and quantitative representation of the defective eyes upon ectopic expression of Not3, Not3-ΔCC, and Not3-ΔNB on wild-type flies with reduced Not3 expression. The mean number of eyes screened is shown on the graph (Y-axis), and three independent crosses were established. Bars show the percentage of eyes screened: blue, normal eyes; light orange, eyes classified as “almost rescued”; dark orange, eyes classified as “not rescued”; *** p < 0.001. In green color, comparisons to UAS-GFP control flies; black color, comparison to UAS-LacZ control flies. Microscopy images show the eyes of adult flies from each category. Scale bars are 200 μM. e Qualitative and quantitative representation of the defective eyes upon ectopic expression of Not3, Not3-ΔCC, and Not3-ΔNB on eyeful flies. Bars show the percentage of eyes screened: blue, normal eyes; light orange, eyes classified as score 1 (presence of 1 fold); dark orange, eyes classified as score 2 (presence of 2–4 folds); ns, means not significant, ** p > 0.01, *** p < 0.001. The mean number of eyes screened is shown on the graph (Y-axis). In green color, comparisons to UAS-GFP control flies; black color, comparison to UAS-LacZ control flies. Microscopy images show the eyes of adult flies from each category. Scale bars are 200 μM. f Quantitative representation of the whole eye area (region with presence of differentiated photoreceptors) on representative adult eyes (n = 8) with ectopic expression of Not3 wild-type or Not3 mutants on the eyeful genetic background. Control eyes measured values were considered as 100%; ns, not significant; * p < 0.05; ** p < 0.01

Back to article page