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Fig. 2 | Journal of Hematology & Oncology

Fig. 2

From: The CCR4-NOT complex is a tumor suppressor in Drosophila melanogaster eye cancer models

Fig. 2

Reduced expression of Not3 in a wild-type genetic background abolishes normal retinal differentiation. a Microscopy images show eyes of adult flies with Not3 reduced expression levels from representative genotypes, scale bars are 200 μM. The percentage of F1 “small rough” defective eyes and the mean number of eyes screened are indicated on the bottom side of each image. Three independent crosses were performed for each experimental condition. b qPCR analyses showing the Not3 expression levels from each genotype. Expression values are calculated from three independent crosses. From each cross, 30 eye-antennal imaginal discs were dissected. The red dotted bar represents the expression value = 1. c Quantification of BrdU, pH3, and cleaved-DCP1a positive cells on the posterior portion of eye-antennal imaginal discs (n = 10). Representative confocal images of eye-antennal imaginal discs of the indicated genotypes. Green staining: BrdU, pH3, or cleaved-DCP1a positive cells; red staining: ELAV protein. Scale bars are 70 μM; ns, not significant; *** p < 0.001. d Microscopy images show eyes of adult flies with downregulation of the Pop2 and twin CCR4-NOT complex subunits on a wild-type background, scale bars are 200 μM. The percentage of F1 “small rough” defective eyes and the mean number of eyes screened are indicated on the bottom side of each image. e Quantitative representation of the whole eye area (region with presence of differentiated photoreceptors) on representative adult eyes (n = 8) with downregulation of Not3 or twin on the wild-type genetic background. Control eyes measured values were considered as 100%; ns, not significant; ** p < 0.01

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