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Fig. 3 | Journal of Hematology & Oncology

Fig. 3

From: Targeting WEE1 to enhance conventional therapies for acute lymphoblastic leukemia

Fig. 3

AZD-1775 reduces the cell viability of primary leukemic samples. a Cell viability analysis on primary leukemic cells isolated from 13 adult ALL patients treated with AZD-1775 (2.5, 5, and 10 uM) for 24 h. Viable cells are depicted relative to the untreated controls. b Quantitative mRNA analyses of 24 genes of the G2/M checkpoint. The basal gene expression of MNCs samples (n = 3) was compared with the basal gene expression of poor (n = 3), intermediate (n = 2), and high (n = 2) responders to AZD-1775 (ex vivo). Clustergram with a color indicative of the degree of upregulation (red) or downregulation (green). Targets with similar regulation cluster together. c Immunoblotting analyses of primary leukemic cells (n = 2, samples #1 and #6) and MNCs (n = 1, donor 4) treated with AZD-1775 (2.5, 5, and 10 uM) for 24 h and then stained for markers of WEE1 functional inhibition (phospho-CDC2) and induction of DNA damages (phospho-CHK1 and phospho-γH2AX). β-actin was used for loading normalization. d Light microscopy analysis of normal MNCs and primary leukemic cells treated with AZD-1775 (10uM) for 24 h and then stained with May-Grünwald Giemsa solutions. In the figure, the yellow arrows indicate DNA bridges induced by the treatment. Controls in all panels are cells treated with DMSO 0.1%. Representative images are shown at × 100 magnification

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