Fig. 5
From: Control of triple-negative breast cancer using ex vivo self-enriched, costimulated NKG2D CAR T cells
CD25 high expression on GFP-NKG2D-z CAR T cells is essential for enrichment in the presence of IL-2. a One representative donor NKG2D CAR T cells expressed high level of CD25 and low level of CD25 by depleting human CD25 with magnetic separation on day 5 (4 days post transduction). b Percentages of CD25high and CD25low expressing GFP+ (NKG2D CAR) cells, presented as the mean ± SD, were derived from three independent donors during a 3-week culture period in the presence of IL-2 (50 IU/ml). c The kinetic monitoring of CD25high and CD25low expressing GFP (NKG2D CAR) expression on one representative donor of three in the presence of IL-2