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Fig. 6 | Journal of Hematology & Oncology

Fig. 6

From: Exosomes released by hepatocarcinoma cells endow adipocytes with tumor-promoting properties

Fig. 6

HepG2 exosomes activate several kinases and NF-κB in adipocytes. a Phospho-kinase antibody array was performed on protein lysates from adipocytes treated with or without HepG2 exosomes. Data (right) are reported as percentage of increase. The percentage was calculated as (exosome − control)/exosome × 100%, and percentage over 20% is considered statistically significant. The top 5 kinases with an increased phosphorylation were highlighted by red boxes in the left panel. b Phosphorylation of AKT, ERK1/2, STAT5α, and GSK3β was confirmed by Western blot. GAPDH was used as loading control. c Representative immunofluorescence staining images of nuclear translocation of p65 in HepG2 exosome-treated adipocytes. Red (anti-p65 antibody), blue (Hochest). d Relative mRNA expression of IL-6, IL-8, and MCP-1 in adipocytes treated with exosome in the presence or absence of NF-κB inhibitor (*P < 0.05, **P < 0.01)

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