Fig. 4

Nano-sar inhibits the Src signaling pathways, migration, and invasion in HNSCC cells. a The effects of saracatinib (Sar) and Nano-sar on Src phospho-activation and the downstream pathways. b The effects of Sar and Nano-sar on colony formation of HNSCC cells within 2 weeks. In this assay, colonies with more than 50 cells were scored and counted under the microscope. c, d The effects of Sar and Nano-sar on migration and invasion of HNSCC cells within 24 h. In b–d, the representative images and quantitative data were shown in the left and right panels, respectively. e The effects of Sar and Nano-sar on 3D invasion in Matrigel within 72 h. *p < 0.05; **p < 0.01