Fig. 1

Generation of anti-FLT3 CAR. a Schematic diagram of anti-FLT3 CAR structure containing CD8α leader, FLT3L-BD, CD8α hinge and transmembrane domains, and intracellular tandem with 4-1BB and CD3ζ signaling domain; GFP was separated with T2A. b Four days after lentiviral transduction, GFP and FLT3L expression on FLT3L CAR-T cells was measured using flow cytometry. c The ratio of CD4⁺ and CD8⁺ T cells in CAR-T or VEC-T cells was detected using flow cytometry. d CCR7 and CD45RA surface staining was performed on both CAR-T and VEC-T cells. Tcm (central memory, CCR7⁺CD45RA⁻), Tem (effector memory, CCR7⁻CD45RA⁻), Temra (terminally differentiated effector memory, CCR7⁻CD45RA⁺)