Fig. 1

Jurkat cells transfer mitochondria to MSCs when exposed to ara-C or MTX. a Flow cytometry analysis of MitoTracker Red uptake by MSCs (GFP+ gated) cocultured with MitoTracker Red-labeled Jurkat cells after 300 nM ara-C or 100 nM MTX was added for 48 h. b Flow cytometry analysis of MitoTracker Red uptake by Jurkat cells (GFP− gated) cocultured with MitoTracker Red-labeled GFP+ MSCs after 300 nM ara-C or 100 nM MTX was added for 48 h. c Representative confocal microscopy images show that Jurkat cell-derived mitochondria (Red+) were internalized in MSCs(GFP+). Scale bar, 10 μm. d Representative confocal images show that MSCs received Jurkat cell-derived mitochondria at different time points (1, 2, and 3 days after coculture). Scale bar, 10 μm. e The areas of red foci per field were calculated by ImageJ software. The data are presented as the mean ± S.E.M. of three independent experiments (*P < 0.05; **P < 0.01; t test)