Fig. 2
Ezh2 promotes GC cell proliferation, clonogenicity, invasiveness and sphere-forming capacity. a The Western blotting results show the baseline protein levels of Ezh2 in GES-1 cell line and five GC cell lines and the efficiencies of Ezh2 overexpression in MKN-45 and SGC-7901 cells and Ezh2 knockdown in AGS cells. *P < 0.01. b The CCK-8 assays showed that overexpression of Ezh2 promoted cell proliferation in MKN-45 and SGC-7901 cells, while knockdown of Ezh2 suppressed cell proliferation in AGS cells. Data are represented as mean ± SEM. *P < 0.01. c The colony-forming assays showed that overexpression of Ezh2 promoted cell colony-forming in MKN-45 and SGC-7901 cells, while knockdown of Ezh2 suppressed cell colony-forming in AGS cells. Data are represented as mean ± SEM. *P < 0.01. d Ezh2-knockdown AGS or scrambled-transfected AGS cells were injected into nude mice (n = 5) subcutaneously (3 × 106 per mouse). The nude mouse xenograft model showed that knockdown of Ezh2 decreased tumor growth (d left) and reduced tumor weights (d middle) compared with the scramble groups. Data are represented as mean ± SEM. * P < 0.01. The representative images of tumors were graphed (d right)