Fig. 2

LIN28B regulates cell cycle progress of AML cells. TF-1a cells were treated either with Scramble shRNA or LIN28B shRNA3 and 5, then grown with BrdU for 12 h stained with aqua dye prior fixation and addition of anti-BrdU APC and 7-AAD as described in Materials and methods section. a Representative images of how viable cells were identified, gated and plot as pseudocolour dot-plots with APC versus 7-AAD. b Representative data showed the distribution of cells at each stage of the cell cycle (in percentage) determined by flow cytometric analysis. This experiment was duplicated. (n = 2, mean ± SD, *p < 0.05; **p < 0.01). c The cell lysates extracted from TF-1a-Scramble shRNA, TF-1a-LIN28B-shRNA3, and TF-1a-LIN28B-shRNA5 were subjected to Western blot analysis for Cyclin D1, Cyclin B1, p21waf1, and beta-actin. Beta-actin was used as internal control