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Fig. 3 | Journal of Hematology & Oncology

Fig. 3

From: The ubiquitin-conjugating enzyme UBE2O modulates c-Maf stability and induces myeloma cell apoptosis

Fig. 3

UBE2O modulates the degradation of c-Maf but not MafA. a HA-tagged c-Maf and Myc-tagged UBE2O plasmids were co-transfected into HEK293T cells for 48 h (left panel) or indicated time points (right panel). Whole cell lysates were subjected to immunoblotting assay against specific antibodies. GAPDH was used as an internal control. b HA-tagged MafA and Myc-tagged UBE2O plasmids were co-transfected into HEK293T cells for 48 h (left panel) or indicated time points (right panel). Whole cell lysates were subjected to immunoblotting assay against specific antibodies anti-HA for MafA and anti-Myc for UBE2O. GAPDH was used as an internal control. c HA-tagged c-Maf and Myc-tagged UBE2O were co-transfected into HEK293T cells; 24 h later, cells were treated with cycloheximide (CHX) for indicated periods. Cell lysates were subjected to immunoblotting assay against HA (c-Maf). d HA-c-Maf plasmids were transfected alone or together with Flag-Ub, Myc-UBE2O into HEK293T cells. Twenty-four hours later, cells were treated with 20 μM of MG132 for 4 h. Cell lysates were subjected to immunoblotting assay against indicated proteins

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