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Fig. 1 | Journal of Hematology & Oncology

Fig. 1

From: The ubiquitin-conjugating enzyme UBE2O modulates c-Maf stability and induces myeloma cell apoptosis

Fig. 1

UBE2O interacts with c-Maf protein. a UBE2O protein was identified by MS in the c-Maf co-immunoprecipitated complex. The unique peptides found in the c-Maf IPs were underlined and highlighted in red. b A typical MS spectrum (SGYPDIGFPLFPLSK) of UBE2O. c, d The HA-tagged c-Maf plasmid and the Myc-tagged UBE2O plasmid were co-transfected into HEK293T cells for 48 h. Lysates were precipitated with an anti-HA or anti-Myc antibody followed by immunoblotting with specific antibodies as indicated. Total cell lysates (TCL) were subjected to immunoblotting assay as control. e Myeloma cell line LP1 was infected with lentiviral UBE2O for 72 h, followed by cell lysate preparation and IP assay

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