Fig. 3

Co-localization of different anti-PTPRG antibodies on the same histo-cytologic structures: murine cerebral cortex (a–d) and vessel sections (e–h) were analyzed using TPγ B9-2 and chPTPRG antibodies. White arrow, a dendrite staining positive for chPTPRG and negative with TPγ B9-2 (b). A slightly different subcellular distribution is also observed in small vessels as shown by overlapping of both stains (in colocalization and merge mode, c, d, g, h). In colocalization mode, yellow regions indicate only the overlapping epitopes. Merge mode shows signals from the individual antibodies highlighting the capability of chPTPRG (in red) to identify dendrites that are not recognized by TPγ B9-2 (in green). Slight differences in staining are present also in vessel sections. These results confirm the presence of different Ptprg isoforms recognized by the antibodies and localized in different cellular structures. DAPI (blue) stains nuclei. Scale bars, 30 μm in a–d, 10 μm in e–h