Skip to main content
Fig. 1 | Journal of Hematology & Oncology

Fig. 1

From: A new monoclonal antibody detects downregulation of protein tyrosine phosphatase receptor type γ in chronic myeloid leukemia patients

Fig. 1

TPγ B9-2 specifically recognizes PTPRG. Downregulation of PTPRG by siRNA demonstrates the specificity of TPγ B9-2 monoclonal antibody for the antigen. Immunoblotting was performed with the indicated antibodies after transfection with a specific PTPRG siRNA (siRNA) and with a negative control siRNA (scrambled: SCR). Cell lines were DBTRG and K562, and antibodies were Mab TPγ B9-2 or the reference rabbit polyclonal antibody RbtP4 and chicken (ch) anti PTPRG [9, 10]. a Immunoprecipitation of PTPRG by TPγ B9-2 monoclonal antibody. K562 and DBTRG cell lines, respectively negative and positive for PTPRG mRNA expression, were subjected to immunoblotting analysis with chPTPRG antibody after immunoprecipitation with TPγ B9-2 antibody. Left side: Black arrow, full-length PTPRG; gray arrows indicate putative processed forms. No signal was detectable using an irrelevant antibody for IP (data not shown). b Western blotting with mab TPγ B9-2 or the reference rabbit polyclonal antibody RbtP4 in PTPRG expressing DBTRG cell line treated with scrambled (SCR) or PTPRG-specific siRNA. Both antibodies detect the downregulation of PTPRG. Anti-β-actin was used as a loading control. c Western blotting with mab TPγ B9-2 or the reference rabbit polyclonal antibody RbtP4 in PTPRG silenced K562 cell lines overexpressing PTPRG (K562 PTPRG+). Downregulation of the 180 kDa band is apparent in silenced cells using both antibodies. Differences in signal intensities are due to the combined effect of individual affinities of primary antibodies toward the native or cDNA-transfected antigens and secondary antibodies toward the murine or rabbit Igs

Back to article page