Fig. 4

miR-330-3p promotes tube formation, migration, and invasion in HUVECs co-cultured with NSCLC cells. a HUVECs were seeded on top of extracellular matrix, in the presence of conditioned medium (CM). Photographs are representative of three independent experiments (original magnification ×100). b HUVECs were seeded in 6-well plates, in the presence of CM. Wound closure was determined 6 and 12 h after the scratch. c Cell invasion was evaluated using a 24-transwell chamber with 8-μm pore insert. HUVECs were seeded in the upper chamber, in the presence of CM. The migrated cells were crystal violet-stained and assessed using an inverted light microscope (original magnification ×100). CM was obtained from NC-LV, OE-miR-330-3p-LV, or anti-miR-330-3p-LV-transfected H460 and H1975 cells. d The expression of VEGFA in H460 and H1975 cells after different treatment by western blotting. Error bars indicate mean ± SD, a representative experiment of three was reported; *P < 0.05, **P < 0.01, #P < 0.001, P value was calculated by one-way ANOVA