Fig. 3

Analysis of thymic tissues. a Schematic diagram displaying the approach used for thymic analysis. b A representative photograph of thymi derived from treated mice. c Representative flow cytometry assessing the GFP frequency of treated vs. ctl mice. d Absolute numbers of total (top) or GFP⁺ (bottom) thymocytes. e Absolute counts of all thymic subsets. f Absolute counts of ETP (top panel), DN2 (middle panel), and DN3 (bottom panel) thymocytes. g Absolute counts of total (top panel), cortical (middle panel), and medullary (bottom panel) TECs. All shown experiments were conducted at least three times with *P < 0.05 and ***P < 0.0001 and n = 10/group