Fig. 1

Peripheral T cell reconstitution. a Schematic cartoon summarizing the BMT protocol. Absolute counts of GFP⁺ (b), GFP⁺CD19⁺ (c), or GFP⁺CD3⁺ (d) T cells in peripheral blood of transplanted mice (n = 10/group). The red dashed line within the pink-shaded area represents the mean physiological level of the GFP⁺ population of interest being analyzed +1 standard deviation calculated using blood samples collected from 10 unirradiated RAG2p-GFP mice. The treatment groups are represented as follows: PBS (empty circle), IL-7 (filled triangle), and IL-21 (filled circle). e Absolute counts of GFP⁺CD4⁺, GFP⁺CD8⁺, NK⁺, or GFP⁺NKT⁺ cells at 5 and 8 weeks post-BMT (n = 10/group). The PBS group is represented by white bars, IL-7 by gray bars, and IL-21 by black bars. f Pie charts reflecting the percentages of all major blood-circulating immune populations. The represented groups are follows: lymphocytes in dark red (68–83%), granulocytes in pink (14–26%), monocytes in yellow (3–5.1%), and eosinophils in light pink (2.5–5.5%). Control mice (Ctl) represent unirradiated mice. g A representative BMT experiment conducted in IL-7^−/− (on a C57BL/6 background) recipient mice (n = 10/group). The treatment groups are represented as follows: PBS for RAG2p-GFP-derived BM → IL-7^−/− (empty circle), IL-21 (50 μg/kg) for RAG2p-GFP-derived BM → IL-7^−/− (filled triangle), and a technical control consisting of PBS administration to RAG2p-GFP-derived BM → WT C57BL/6 setting (filled circle). All experiments were conducted at least three times with *P < 0.05, **P < 0.01, and ***P < 0.0001