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Fig. 1 | Journal of Hematology & Oncology

Fig. 1

From: Cellular function reinstitution of offspring red blood cells cloned from the sickle cell disease patient blood post CRISPR genome editing

Fig. 1

Preparation of HSPCs from the peripheral blood of SCD patients and CRISPR/Cas9 genome editing. a Immunophenotyping analysis of HSPCs in patient whole blood by flow cytometry. b Immunophenotyping of isolated CD34+ HSPCs. c Morphological examination of CD34+ HSPCs and CD34-blood-nucleated cells with Wright-Giemsa stain. d Cell counts of isolated HSPCs pre- and post-expansion. e Colony formation of HSPCs post CRISPR genome editing. f Counts of formed E- and G/M-colonies per milliliter of the patient peripheral blood

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Journal of Hematology & Oncology

ISSN: 1756-8722

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