Fig. 5

Blimp-1 knockdown with siRNA increases cytokine production and cytotoxicity capacity in T cells from AML patients. a Histograms of Blimp-1 MFI show the efficiency of Blimp-1 siRNA knockdown. b Intracellular cytokine production by purified CD4⁺ and CD8⁺ T cells from AML patients (n = 4) upon anti-CD3/anti-CD28 stimulation. c Intracellular production of perforin by purified CD8⁺ T cells from AML patients (n = 4) upon Blimp-1 knockdown. Shown are representative flow data (left) and plot of frequency (right). P values were obtained by paired t test.