Fig. 1

Blimp-1 is elevated in T cells from AML patients. PBMCs collected from AML patients at initial diagnosis as well as healthy donors were assessed. a, b Expression of Blimp-1 mRNA was assessed by SmartFlare probe followed by flow cytometry analysis. a Representative histograms displaying the expression of Blimp-1 mRNA gated on CD4⁺ (left) and CD8⁺ T cells (right). Data from one healthy donor and one AML patient are shown. Scrambled SmartFlare probes were set as negative controls. b Plot of percentages of Blimp-1⁺ cells in CD4⁺ and CD8⁺ T cells from healthy donors (n = 25) vs. AML patients (n = 24). c, d Expression of Blimp-1 protein was assessed by intracellular staining. c Representative flow cytometry data. d Plot of percentages of Blimp-1⁺ cells in CD4⁺ and CD8⁺ T cells from healthy donors (n = 19) vs. AML patients (n = 14). Each spot represents an individual patient or healthy donor. P values were obtained by unpaired t test.