Fig. 3

The GTP-binding, not transamidating, activity of Gαh determines the Gαh-induced invasiveness of TNBC cells. a Western blot analysis for Gαh, GTP-bound Gαh, and GAPDH expression in HCC1806 cells transfected with vector control (VC) or exogenous Gαh without (wild-type, wt) or with mutations at residues 241 (W to A) and 508 (R to A). The levels of GTP-bound Gαh from three independent experiments were normalized as fold changes relative to VC group and presented as mean ± SEM (bottom). b Transamidating activity assay of HCC1806 cell variants. c and d Giemsa staining c and quantification d of invaded cells after 16 h of incubation of HCC1806 cell variants. e Western blot analysis for Gαh, GTP-bound Gαh, and GAPDH expression in MDA-MB-231 cells transfected with non-silencing (NS) shRNA or Gαh-targeting shRNA followed by restoration of the expression of VC or exogenous wild-type and mutant Gαh. The levels of GTP-bound Gαh from three independent experiments were shown as fold changes relative to NS group and presented as mean ± SEM (bottom). f Transamidating activity assay of MDA-MB-231 cell variants. g and h g Giemsa staining and h quantification of invaded cells after 16 h of incubation of MDA-MB-231 cell variants. In a, b, d, e, f, and h, different letters above the columns indicate significant differences between the means (p < 0.05)