Fig. 3

The frequency of regulatory T cells is increased in CD4⁺ T cells developing in NSG mice following intrathymic injection of hematopoietic stem/progenitor cells. NSG mice were intrathymically injected with 10,000 C57BL/6 LSK cells. Spleens were harvested on day 56 and CD4⁺ T cell subsets were analyzed by flow cytometry. a Splenic CD25⁺FoxP3⁺ regulatory T cells (frequencies of CD4⁺ T cells) in intrathymically injected NSG mice and NOD control, untreated mice. Combined data from two independent experiments are shown. Mean and SEM are presented (n = 5–10). b The frequencies of Helios⁺ natural regulatory T cells that were generated by intrathymically injected NSG mice. Mean and SEM are presented (n = 9). c The ratio of CD4⁺ effector (CD62L⁻CD44⁺)/regulatory T cells in NOD control mice and intrathymically injected NSG mice. Mean and SEM are presented (n = 5–13)