Fig. 7

RUNX2 may facilitate SUMO-deficient PR target gene expression. a SLC37A2 genomic region contains multiple RUNX binding motifs and other regulatory regions (CpG islands and other transcription factor binding hot spots). The number (#) of RUNX binding motifs within three major regions are listed. b SLC37A2 expression in T47D cells was measured after treatment with progestin (R5020) by RT-qPCR. c SLC37A2 expression in MCF-7 cells was measured after treatment with progestin (R5020) and/or antiprogestin (mifepristone) by RT-qPCR. d SLC37A2 expression in BT474 cells was measured after treatment with progestin (R5020) and/or antiprogestin (onapristone) by RT-qPCR. e T47D cells expressing WT or KR PR were engineered to stably express shRNAs targeting RUNX2, resulting in approximately 50% reduction of RUNX2 mRNA levels. f In cells stably expressing shRNAs targeting RUNX2, expression of the KR PR target gene SLC37A2 was significantly reduced