Fig. 5

LncRNA uc.134 represses HCC proliferation and metastasis via the uc.134-CUL4A-Hippo axis. a Western blot showing that CUL4A abrogated the uc.134-mediated activation of Hippo kinase signaling (left), whereas the knockdown of CUL4A reversed the si-uc.134-mediated repression of LATS1 and inactivation of YAP (right). b A Transwell analysis showed that CUL4A overexpression abrogated the uc.134-mediated repression of HCC cell invasion (upper), whereas CUL4A knockdown yielded the opposite results (lower). All experiments were performed in triplicate, and results are presented as mean ± SD. ***P < 0.001. c A CCK8 proliferation analysis showed that the transfection of CUL4A plasmid or LATS1-specific siRNA abolished the uc.134-mediated growth inhibition (left), whereas the transfection of siCUL4A or LATS1 plasmid yielded opposite results (right). The mean ± SD is shown for five independent experiments. **P < 0.01; and ***P < 0.001