Fig. 2

ELF2B overexpression decreases cellular proliferation and clonogenicity in vitro. a Number and distribution of somatic mutations in ELF2A and ELF2B compiled from TCGA and COSMIC databases (see Additional file 6: Table S4). b RNAseq expression analysis of ELF2 from 30 TCGA studies; data is represented with box and whisker plots, showing quartiles and minimum and maximum values. Expression is in RNASeq V2. c RNAseq expression of ELF subfamily members in acute myeloid leukaemia (AML) compared to all other cancers (29 in total) from TCGA data. Expression is in RNASeq V2 (log). d Schematic of ELF family members showing the conserved Ets DNA-binding domain, conserved acidic domains A–D and known protein interaction domains for RB1, RUNX1, and LMO2. Amino acid similarity scores between ELF2A and all ELF proteins are indicated. e Overexpression of ELF proteins in HeLa and HFF cells: with control (GFP empty vector, Con) and HA-tagged ELF protein-containing lentivectors. MTT proliferation assay in HeLa (f), and HFF cells (g). Clonogenicity assay in HeLa (h) and HFF cells (i). Representative images of Giemsa-stained colonies are shown. Data represents the mean ± SEM of three experiments each performed in triplicate with statistical analysis performed using Mann-Whitney U test (ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001). Statistical significance is indicated relative to GFP control