Fig. 4

Ectopic expression of miR-195-5p inhibits proliferation and colony formation of DLD1 and HCT116 cells. Representative photomicrographs (a) and quantifications (b) of BrdU staining in DLD1 and HCT116 cells after transfection with miR-195-5p mimic, miR-195-5p mimic NC, miR-195-5p inhibitor, or miR-195-5p inhibitor NC for 48 h. Bar = 100 μm. Representative photomicrographs (c) and quantifications (d) of the colony formation assay after transfection with miR-195-5p mimic, miR-195-5p mimic NC, miR-195-5p inhibitor, or miR-195-5p inhibitor NC for 8 days. CCK8 cell proliferation assays in DLD1 (e) and HCT116 (f) cells transfected with indicated miRNAs. Assays were performed in triplicates. Mean ± SD is shown. Statistical analysis was conducted using one-way ANOVA. *P < 0.05. **P < 0.01. ***P < 0.001