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Fig. 3 | Journal of Hematology & Oncology

Fig. 3

From: Pterostilbene prevents AKT-ERK axis-mediated polymerization of surface fibronectin on suspended lung cancer cells independently of apoptosis and suppresses metastasis

Fig. 3

PS triggers apoptosis of LLC cells in adherent, but not suspended, status. a Suspended LLC cells treated with DMSO or various concentrations of PS in a time course (0–24 h) were stained with Hoechst and incubated with PI at each time point as indicated in centrifugation tubes. % cell viability was calculated as 1-PIhigh cell number/ Hoechsthigh cell number (left panel). Right panel: representative images showing the co-staining of Hoechst and PI in PS-treated suspended cells at the 24-h time point. b Adherent LLC cells were similarly treated with DMSO or PS and stained as in (a) within the culture dishes. Cell death % was calculated as PIhigh cell number/ Hoechsthigh cell number (left panel). Right panel: representative images showing the co-staining of Hoechst and PI in PS-treated adherent cells at the 24-h time point

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