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Fig. 1 | Journal of Hematology & Oncology

Fig. 1

From: Incorporation of a hinge domain improves the expansion of chimeric antigen receptor T cells

Fig. 1

Hinge incorporation can promote the expansion of CAR T cells. a Schematic representation of CAR constructs specific for different antigens, and with or without a hinge domain. Flow cytometric analysis of the percentage of (b) 19.28z, 19-H.28z T cells, (c) Meso.28z, Meso-H.28z T cells, (d) PSCA.28z, PSCA-H.28z T cells, and GFP control T cells from day 6 to 15 during the in vitro culture period. The data are representative of independent experiments verified with cells from over three individual healthy human donors. Total cell number of (e) 19.28z T and 19-H.28z T cells, (f) Meso.28z T and Meso-H.28z T cells, (g) PSCA.28z T, and PSCA-H.28z T cells from day 4 to day 16 during the in vitro culture period. Error bars denote the SEM, and the results were compared through an unpaired t test. *P < 0.05, **P < 0.01, and ***P < 0.001. Flow cytometric analysis of the percentage of (h) 19.28z T, Meso.28z T, HER2.28z T cells, (i) PSCA-H.28z T, MUC1-H.28z T, and GFP control T cells from day 6 to 21 during the in vitro culture period. The data are representative of independent experiments verified with cells from over three individual healthy human donors

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