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Fig. 2 | Journal of Hematology & Oncology

Fig. 2

From: Targeting the CXCR4 pathway using a novel anti-CXCR4 IgG1 antibody (PF-06747143) in chronic lymphocytic leukemia

Fig. 2

PF-06747143 binds specifically to human CXCR4-expressing cells and blocks CXCL12-induced calcium flux. a CHO-parental and CHO-hCXCR4 cell lines were exposed to 20 μg/mL of either a human IgG1 ĸ-PE antibody (isotype control) or PF-06747143-PE and analyzed by flow cytometry. b Calcium flux assay was performed in human T cell leukemia Jurkat cells incubated with PF-06747143, m15-IgG1, or isotype control IgG1 antibody in presence of CXCL12 at 8 nM. Experiment was performed in quadruplicates. Shown are mean intracellular calcium concentrations in relative fluorescence units (RFU). ± standard error of the mean (SEM)

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