Studying hematopoiesis using single-cell technologies

Table 1 Classification of single-cell analysis methods

Method	Amplification	Coverage	References
Genomics
MDA	MDA	High coverage	[30]
MALBAC	MALBAC	High coverage	[31]
Transcriptomics
Single-cell qPCR	Multiplexed PCR	Target gene	[16]
Tang-seq	PolyA tailing + second-strand synthesis	Full-length mRNAs	[34]
CEL-seq	In vitro transcription	3′ End of mRNA	[21]
Smart-seq	Template switching	Full-length mRNAs	[36]
Cyto-seq	Multiplexed PCR	3′ End of mRNA	[23]
Drop-seq	Template switching	3′ End of mRNA	[24]
inDrop	In vitro transcription	3′ End of mRNA	[25]
Proteomics
Mass cytometry	NA	Target protein	[55]
Epigenomics
scATAC-seq	Adaptor PCR	Accessible DNA regions	[64]
scRRBS	Adaptor PCR	1.5 million CpG sites	[59]
scHi-C	Adaptor PCR	NA	[63]
scChIP-seq	Adaptor PCR	About 1000 peaks	[62]

ISSN: 1756-8722