Fig. 4
From: NEK2 Promotes Aerobic Glycolysis in Multiple Myeloma Through Regulating Splicing of Pyruvate Kinase
NEK2 is a transcriptional target of c-Myc. a Dot-plots present the expression of NEK2, c-Myc, and PKM2 in plasma cells of GEP derived from normal donors (n = 22), MGUS patients (n = 44), low- (n = 305), and high- (n = 46) risk MM patients (p < 0.001). b ChIP PCR detected binding of c-Myc to the promoter of Nek2 in P493-6 cells. IgG antibodies were used as negative control. c Real-time PCR shows the expression of NEK2 in P493-6 cells after silencing c-Myc. d Western blots show protein expression of NEK2, c-Myc, and β-actin in P493-6 cells with knocking down c-Myc. e Kaplan-Meier analyses of event-free survival (top panels) and overall survival (bottom panels) among MM patients with different expression levels of NEK2 and PKM2