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Fig. 1 | Journal of Hematology & Oncology

Fig. 1

From: NEK2 Promotes Aerobic Glycolysis in Multiple Myeloma Through Regulating Splicing of Pyruvate Kinase

Fig. 1

NEK2 interacts with hnRNPA1/2 proteins. a Western blots confirm that NEK2 cDNA conjugated with tags HA-3xFLAG are transfected into the myeloma cell line ARP1. b Immunofluorescence analysis of ARP1 cells stained with NEK2 antibody (red), hnRNPA1/2 (green), and DAPI (blue). c HA antibody was used to pull down NEK2, and its interacting proteins were analyzed by Western blotting. The lysates before IP were used as a positive control and IgG pulled down proteins as a negative control

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