Fig. 2

Morphological analysis and roughness measurements of shENO1 cells. a shCTRL and shENO1 CFPAC-1 cells stained for nuclei (DAPI, blue fluorescence), actin (Phalloidin-TRITC, red fluorescence), and ENO1 (anti-ENO1 mAb followed by anti-mouse FITC, green fluorescence). Panels represent images taken at the level of nuclei. b Images taken at the level of the cell surface. c Three-dimensional topographic images of shCTRL and shENO1 CFPAC-1 performed through the AFM technique (three representative images for each group) (scan size: 50 μm; Z scale 4 μm). d Topographic images of shCTRL and shENO1 CFPAC-1 performed through the AFM technique. Left panels: height parameter; central panels: deflection parameter; right panels: magnification of deflection panel. e Histograms represent roughness analysis. A representative of three independent experiments is shown. Data are mean ± SEM. ***p < 0.001 relative to control cells