Fig. 4

XIAP BIR domain promoted miR-200a transcription by inhibiting c-Jun protein phosphorylation at Ser63/73. a miR-200a promoter luciferase activity was evaluated by the Dual-Luciferase Reporter Assay System. Results are the mean ± SD of triplicates. The asterisk “*” indicates a significant increase as compared with nonsense cells (p < 0.05). The symbol “※” indicates a significant inhibition as compared with vector transfectant (p < 0.05). b The diagram of predicted transcription factor binding sites in miR-200a promoter region. c Western blotting was used to analyze the transcription factors expression, and GAPDH was used as the protein loading control. d Short hairpin RNA-specific targeting human RelB were stably transfected to UMUC3(shXIAP) cells, and Western blotting was used to determine the knockdown efficiency and EGFR expression, while GAPDH was used as the protein loading control. e p100 was transiently transfected to T24T cells, and Western blotting was used to determine the expression of p100 and EGFR, and β-actin was used as the protein loading control. f TAM67 was stably transfected into UMUC3 cells, and real-time PCR was used to determine the miR-200a expression. Results were presented as the mean ± SD of triplicates. The asterisk “*” indicates a significant inhibition as compared with vector transfectants (p < 0.05). g The cell extracts from UMUC3(Vector) and UMUC3(TAM67) were subjected to Western blot for determination of the indicated protein expression, and GAPDH was used as the protein loading control