Fig. 2

MAGL enhances HCC cell growth and invasiveness in vivo and in vitro. a, b The protein and mRNA levels of MAGL in different HCC cell lines (HepG2, SMMC7721, Huh7, MHCC97L, MHCC97H, and HCCLM3) and one normal liver cell line (L0-2). c, d Western blot and qRT-PCR analysis showed the protein and mRNA expression of MAGL in HCC cells (HepG2-vector, HepG2-MAGL, HCCLM3-vector, and HCCLM3-shMAGL). e MAGL overexpression promoted HCC progression in vivo. Xenograft model of HCC in nude mice were established as described in the “Methods” section. The tumor volumes of subcutaneous tumors between the experiment and control groups exhibited significant differences. f The motility of HCC cells was detected by scratch wound assay, and statistics are shown with a bar graph. g The invasion ability of HCC cells was measured by transwell Matrigel invasion assay, and statistics are shown with a bar graph. Data, mean + SD. **P < 0.01 and ***P < 0.001